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White Papers and Videos

Automate Your SARS-CoV-2 S1/RBD IgG Ab ELISA and Increase Your Sample Throughput

White Paper

The Freedom EVOlyzer is a dedicated platform that automates all common ELISA steps, including sample distribution, sample predilution, reagent pipetting, incubation, plate washing, optical density reading, and result generation.

This application note compares the results of manual and automated SARS-CoV-2 ELISA workflows and evaluates cross-contamination risk, assay drift, precision, and time to results, finding the automated assay to perform comparably to the manual workflow while increasing throughput.

Quantifying the Impact of Pipette Tip Type Using Dual Dye Ratiometric Technology

White Paper

The type, characteristics, and quality of disposable pipette tips can directly affect the volume transferred to and from an assay, regardless of operator skill or type of pipette employed. This application note from Artel describes the use of a dual-dye photometric technology to correlate the accuracy and repeatability of volume transfers with the disposable pipette tips employed, allowing users to screen and select the appropriate tip types for their liquid handlers, pipettes, and assays.

Pipetting Viscous Solutions – Best Practices

White Paper

This reference poster from Artel describes best practices for pipetting viscous solutions including using positive displacement for methods or reverse mode pipetting.

Artel Lab Reports

White Paper

This ebook from Artel contains a series of publications that provide technical information regarding the use, application, and metrology related to liquid handling instrumentation, including practices for pipette calibration, pipetting technique, and pipette use ergonomics.

Troubleshooting Automation

White Paper

This reference poster from Artel describes how to troubleshoot problems with automated assays, including how to inspect laboratory instruments, consumables, reagents, and other factors that may be causing issues in data.

HPV-Based Cervical Cancer Screening: Gaining Insights in Sample Preference and Cost-Effectiveness

White Paper

Cervical cancer can be prevented through vaccination and screening, but it remains the fourth most common cancer type in women, with over 500,000 cases detected in 2018. Most cases of cervical cancer are linked to types of high-risk human papillomavirus (HPV), a common sexually transmitted infection. Regular screening for cervical cancer with pap smears improves incidence and mortality rates, but many women are reluctant to undergo a pap smear due to the invasiveness of cervical sampling, cultural reasons, lack of time, and the need to visit a clinician.

This whitepaper from Novosanis reviews studies examining patient preferences and the cost-effectiveness of self-sampling methods for HPV-based cervical cancer screening, highlighting the potential of Colli-Pee — a self-sampling urine-capturing device that collects the first 20 ml of urine flow.

Colli-Pee - Performance of A Game-Changing Sampling Device for HPV-Based Cervical Cancer Screening

White Paper

Cervical cancer causes over 300,000 deaths per year. The introduction of screening programs to detect pre-cancer lesions has resulted in a 50 percent reduction of premature deaths. However, non-attendance rates are high: on average 40 percent of women living in developed countries and 80 percent in developing countries do not participate in screening due to the invasiveness of cervical sampling, cultural reasons, lack of time, and the need to visit a clinician.

This whitepaper from Novosanis explains the performance of Colli-Pee — a self-sampling urine-capturing device that collects the first 20 ml of urine flow — in HPV-based cervical cancer screening, highlighting current studies and literature in the field and demonstrating the device’s use for non-invasive, self-administered screening.

Responding to a Pandemic

White Paper

ABC Labs, based at the Karolinska Institute campus in Stockholm, Sweden, was founded soon after the start of the pandemic to establish large-scale PCR and ELISA COVID-19 testing. The laboratory analyzes thousands of tests daily in partnership with Sweden’s Public Health Agency and regional and private healthcare providers.

This case study brief from Tecan describes how ABC Labs employed robotic workstations to scale up their PCR and serology testing over the course of the pandemic from hundreds of samples a day to nearly 20,000.

10 Strategic Considerations When Outsourcing Production of In Vitro Diagnostics

White Paper

This white paper highlights 10 important benefits of outsourcing and key considerations that should factor into the evaluation of an IVD  partner, from investment to development capabilities.

LGC, Biosearch Technologies Accelerate Probe and Primer Production to Enable COVID-19 Testing with the SP Genevac HT Series 3i Evaporator

White Paper

During the COVID-19 pandemic, LGC, Biosearch Technologies, has been accelerating its production of oligonucleotides and other critical test kit components. However, there are problems that can slow production workflow. One particular bottleneck is in the drying process. The output from the synthesis labs and purification group can far outweigh the aliquoting, loading and drying workflow, which causes workflow to slow down upstream.

LGC, Biosearch Technologies, is using SP Genevac technology in order to facilitate the rapid production of probes and primers, and creating them to the high standards needed in this extremely challenging situation.

Download this white paper to learn how Biosearch Technologies used the SP Genevac technology to step up to the COVID-19 testing challenge.

Using Parallel Evaporators to Escalate COVID-19 Test Kit Preparation

White Paper

As labs globally have scaled up production of PCR test kits for COVID-19, workflow improvements continue to be important to increase the efficiency of test kit production.

SP Genevac's HT Series3i evaporator range offers a solution to improving the efficiency of test kit production, by preventing bottlenecks in high-throughput processing of oligonucleotides. It provides a fast and reliable method for drying primers and probes ready for use in PCR testing kits.

Download this white paper to learn how SP Genevac supported laboratories around the world to scale up their production of PCR test kits to aid the global fight against the COVID-19 virus.

Freeze-Drying COVID-19 Diagnostics: Formulation & Process Development and Lyophilizer Selection

White Paper

Lyophilization may be considered a relatively gentle drying process, but there are still risks and pitfalls when applying it to biomolecular reagents in diagnostic tests. Reagents typically used in ELISA- and PCR-based diagnostic kits for diseases such as COVID-19 tend to contain labile components, such as enzymes that need preserving for longevity in the supply chain or antibodies to test for a patients response to the virus, which can be challenging to stabilize for commercial use.

This technical note discusses various aspects of formulation and cycle development in the lyophilization of diagnostic reagents, including those required for COVID-19 testing.

PCR Diagnostic Kits & Freeze-Drying Considerations

White Paper

Polymerase chain reaction (PCR) is a method widely used in molecular biology to make many copies of a specific DNA segment. There are numerous processing challenges with PCR, however. The various component solutions need to be accurately combined in a plate well or tube "cocktail." The components require refrigeration and they have a relatively short shelf life and cross contamination is a concern. R&D lab bench pipetting errors can also be troublesome.

Freeze-drying has become an integral part of the PCR process and is used for both individual reagents as well as end-product test kit reagents.

This technical note discusses the considerations for freeze-dried PCR reagents and diagnostic kits.

Optimized Lateral Flow Assays Using Dyed and Fluorescent Estapor Microspheres

Video

In this technical presentation, we will discuss the use of polymeric microspheres in diagnostic assays. We will provide you with a survey of the materials used in this field, examples of readers available for this growing market, case studies, and a first preview of novel products to be released in 2020.  Material considerations to be discussed include key attributes needed to select the best spheres for qualitative and quantitative assays (such as size, functional group, and color).  We will also share best practices to troubleshoot protein binding in qualitative assay development using our raw materials. This information will be of interest to individuals involved in developing and validating qualitative and quantitative assays.

Applying AnteoBind Activated Estapor Europium Microspheres for Development of Fluorescent Lateral Flow Immunoassays

Video

In this technical presentation, we will provide you with an introduction to AnteoBind nanocoating technology and the use of AnteoBind activated Estapor europium microspheres in lateral flow immunoassays.

We will discuss the common issues encountered in antibody conjugation, the detail of the alternative protein conjugation procedure using AnteoBind activated Estapor europium microspheres, and the effects of physical characteristics (size, dispersity, surface charge) during the steps of conjugation and storage. We will also discuss the performance of conjugates in functional assay development for several popular biomarkers.

We will share best practices to troubleshoot antibody conjugation to AnteoBind activated Estapor europium microspheres in assay development and the initial scale-up procedure. This information will be of interest to individuals involved in developing and validating lateral flow immunoassays and manufacturing process development.