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White Papers and Videos

Assessment of a Novel Fluidics Platform for Rapid Sample Processing from Whole Blood to Library

White Paper

This poster from Volta Labs describes an evaluation by Broad Clinical Labs of a walk-away, end-to-end DNA extraction to library construction platform from Volta Labs, which employs digital fluidics technology controlling the sample chemistry, using it to create a clinical grade whole genome starting from whole blood.

The Callisto Sample Prep System Supports Robust and Versatile Sample Prep for High-Quality HiFi Long-Read Sequencing

White Paper

Highly accurate (HiFi) long-read SMRT (single-molecule real-time) DNA sequencing on the PacBio platform is now commonly used, particularly for applications where short-read NGS has been proven to be inadequate. These include de novo assembly of haplotype-resolved reference genomes, detection and characterization of large structural variants, interrogation of challenging genomic regions, direct methylation analysis, and metagenome deconvolution. Recent advances in the accuracy, throughput, and speed of HiFi sequencing — combined with a significant reduction in per-base cost — promise to broaden access and accelerate the adoption of long-read sequencing in life sciences research and the clinic. Proportionate improvements in the scalability, robustness, and cost of sample preparation for HiFi sequencing are needed.

This application note from Volta Labs demonstrates the extraction of high-quality high molecular-weight DNA and the preparation of SMRTbell libraries in different reaction formats, from a range of inputs, and with different workflow parameters, generating high-quality HiFi sequencing data and variant calls with minimal training and a significant reduction in hands-on time.

Digital Fluidics Enables Broad-Based Access to Push-Button NGS Sample Prep

White Paper

Decades and billions of dollars have been dedicated to advancing sequencing technologies and genomic data analysis. This has resulted in a reduction in sequencing cost by six orders of magnitude and an explosion in sequencing-based applications. However, the sample preparation process remains a significant bottleneck. When samples are processed manually, variable sample quality, laborious protocols, and high per-sample cost remain significant obstacles to scalability and consistency. Automated liquid handlers enable higher throughput, and process but significant barriers to implementation persist. These include high capital expenditure, the need for multiple, dedicated instruments, specialized infrastructure and/or skills, lengthy method development, and ongoing maintenance costs.

This poster from Volta Labs describes a benchtop automated sample prep system and presents three case studies demonstrating the system’s ability to produce higher yields of quality DNA, produce libraries from lower inputs, and produce slightly better variant calling than standard manual protocols.

Digital Fluidics Simplifies NGS Library Preparation in a Clinical Setting

White Paper

Process control is essential to ensure high success rates and consistency when preparing samples for NGS-based assays. Automation facilitates process control. Conventional liquid handlers are widely employed in clinical laboratories but require a significant investment in equipment, method development, and validation — as well as skilled personnel to operate and maintain. Volta Labs is introducing a reimagined digital fluidics technology that utilizes electrical, thermal, and magnetic fields to enable programmable, precise, and plastic-free liquid manipulation. This enables high-performance, walk-away NGS sample preparation, and opportunities to improve operational efficiency. Pre-optimized methods enable rapid implementation with minimal training.

This poster from Volta Labs describes how authors from Volta and Helix investigated the feasibility and potential benefits of the Volta Labs automated sample prep platform in a clinical setting by automating an established commercial library prep method for PCR-free WGS, finding it to deliver comparable or superior library quality compared to traditional protocols.

Automated Hybridization Enrichment of Hartwig OncoAct Panel Using the Volta Labs Digital Fluidics Platform

White Paper

The Hartwig Medical Foundation is a not-for-profit organization dedicated to providing cancer patients with the best treatment options. As part of this mission, they have developed a WGS-based molecular diagnostic test, OncoAct WGS, which generates a tumor-normal comparison from fresh tumor and normal material and returns a molecular diagnostic report supported by information from knowledge bases and the Hartwig database. Not all samples (e.g., FFPE tissue, fresh biopsies with a tumor cell content of 10-20 percent) are suited for OncoAct WGS. They therefore developed a targeted sequencing assay, OncoAct Panel, to inform treatment options for more patients. The OncoAct WGS workflow is fully automated, and the foundation is working toward a fully automated hybridization capture workflow with the highest possible quality and robustness for standard diagnostic use. The foundation entered into a collaboration with Volta Labs to explore the benefits and feasibility of automating the OncoAct Panel workflow on the innovative digital fluidics platform developed by Volta Labs.

This poster from Volta Labs presents results from a Hartwig Medical Foundation study exploring the automation of the OncoAct WGS-based molecular diagnostic panel using Volta Labs' digital fluidics platform, demonstrating that automated post-hybridization capture and wash steps can achieve or surpass the performance of established manual methods.

Two Industry Leaders on Dealing With the Growing Complexity of Genomic Cancer Data

White Paper

The Clinical Knowledgebase (CKB) powered by The Jackson Laboratory (JAX) is a dynamic, digital resource for interpreting complex cancer genomic profiles in the context of gene variant knowledge and protein impact, therapies, and clinical trials. Illumina has teamed up with JAX in the newly released Connected Insights software, which integrates CKB as a knowledge source.

In this podcast, Mendelspod’s Theral Timpson speaks to Cara Statz from JAX and Erica Schnettler from Illumina about how their combined technologies are helping researchers and oncologists deal with the increasing complexity of cancer genomic data. They discuss oncologists' experience dealing with an overwhelming amount of new scientific data, and both guests give their advice for new labs setting up cancer testing and oncology clinical research.

Constant Performance System Degassing

White Paper

This ebook from IDEX Health & Science discusses the role of in-line vacuum degassing and presents a new method by which vacuum degassing can be controlled such that the efficiency of the degasser can be set to a constant value at any flow rate.

Advanced Spectral Measurement Systems and Semrock Optical Filters

White Paper

The need for increasingly demanding spectral specifications has required new tools capable of corresponding spectral measurements. Key among these specifications are optical blocking levels and spectral edge steepness measured from UV to NIR wavelengths over a wide range of angles.

This white paper summarizes the features of the proprietary spectral measurement tools developed by IDEX Health & Science, including the established Peregrine and the new KolaDeep Spectral Measurement Systems.

Fittings 101: A Practical Guide to Connections and Fittings in the Laboratory

White Paper

This e-book from IDEX Health & Science provides comprehensive guidance on fittings for laboratory instruments, including how to describe a fitting, how to determine where certain styles of fittings are used in a system, the interchangeability of different types of fittings, and the special terminology and skill set needed to work with hyphenated chromatography systems.

Understanding and Testing Fluid Flow for Custom Valve Design

White Paper

There are many factors taken into consideration during the development of a valve design. Prototyping the complex geometries of valve stators can be expensive and time-consuming but simulation tools can help iterate multiple designs quickly and select the most desirable solutions before the first parts are manufactured.

This white paper from IDEX Health & Science discusses the computer simulation and analysis equipment IDEX Health & Science leverages to reduce valve design iterations and risk of unexpected mechanical failures, evaluate valve components to understand the impact of seal wear, and balance cost, delivery, and design for optimized flow path alignment and tolerancing.

Selecting Filters for Fluorescence Multiplexing

White Paper

The steady advances in optical thin film deposition technology over recent decades have enabled the production of high-performance multiband optical filters that address the increasing demand for multicolor fluorescence instrumentation. Though there is now a wide range of available catalog filters designed for a large variety of fluorophores, selecting suitable filters is often a complex process.

This white paper from IDEX Health & Science presents considerations relevant to selecting multiband optical filters when designing a fluorescence imaging system, particularly one with optical multiplexing.

Powering Diagnostics at Scale: Large Custom Projects

White Paper

This e-book from Cytiva describes customer success stories in which Cytiva collaborated with diagnostics developers on large custom projects to provide lyophilized reagents for a point-of-care device, design and build a multiplex lateral-flow assay, provide magnetic beads for a blood screening test, create kits for a cell analysis device, and create a new facility to lyophilize and package reagents for a sequencing platform.

Powering Diagnostics at Scale: Small- to Mid-Size Custom Projects

White Paper

This e-book from Cytiva describes customer success stories in which Cytiva collaborated with diagnostics developers on small- to mid-sized custom projects to scale up a rapid COVID-19 test, customize an early cancer test, improve a food-and-beverage test, assemble a kit for an immunodiagnostic test, and provide quality control for a diagnostic test.

Fast and Reproducible AAV Vector Quantification with Simple Plex Assays on Ella

White Paper

As part of the viral quantification process, researchers rely on various analytical methods to assess critical quality attributes. While traditional plate-based and dot blot assays can be useful, they are often complex, time-consuming, and non-standardized. To meet increased throughput and safety demands, fast and reproducible titration of viral particles is essential.

This application note from Bio-Techne pairs Simple Plex microfluidic cartridge assays on the Ella automated ELISA platform with Progen antibodies to demonstrate utility for accurate and efficient AAV capsid titer determination, showing how the specificity of Progen’s serotype-specific antibodies helps ensure reliable AAV quantification.

Meeting the Challenges of Early Cancer Detection

White Paper

This solution brief from Bio-Techne describes the drawbacks of relying on circulating tumor DNA for non-invasive early cancer detection and how incorporating proteomics can enhance the sensitivity of early detection tests.