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White Papers and Videos

Responding to a Pandemic

White Paper

ABC Labs, based at the Karolinska Institute campus in Stockholm, Sweden, was founded soon after the start of the pandemic to establish large-scale PCR and ELISA COVID-19 testing. The laboratory analyzes thousands of tests daily in partnership with Sweden’s Public Health Agency and regional and private healthcare providers.

This case study brief from Tecan describes how ABC Labs employed robotic workstations to scale up their PCR and serology testing over the course of the pandemic from hundreds of samples a day to nearly 20,000.

Protecting Data Integrity and Quality Assurance in Times of Crisis: Technologies Essential to SARS-CoV-2 Sequencing

Video

Clinical Reference Laboratory (CRL) is one of the largest privately held clinical testing laboratories in the US. As the COVID pandemic arose last year, the lab sought to develop a patient-friendly sample collection experience based on saliva instead of nasal pharyngeal swabs. But the test needed to be just as accurate and reliable as standard SARS-CoV-2 tests.

In this on-demand eCase study, Heather Fehling and Rob Grbac of CRL review how they quickly developed a PCR-based COVID-19 saliva test and then rapidly scaled from 300 samples per day to 30,000 samples per day. They discuss how they optimized their processes with the help of Artel’s Multichannel Verification System and review lessons learned from the project.

Download the transcript that accompanies this eCase Study here.

Protecting Data Integrity and Quality Assurance in Times of Crisis: Technologies Essential to SARS-CoV-2 Sequencing - A GenomeWeb eCase Study Transcript

White Paper

This transcript is a companion piece for a GenomeWeb eCase Study in which Heather Fehling and Rob Grbac of Clinical Reference Laboratory discussed how their lab quickly developed a PCR-based COVID-19 saliva test and optimized their processes with the help of Artel's MVS technology. The transcript of the presentation has been lightly edited for clarity and length.

Avoid False Positive ELISA Data

White Paper

Complex samples such as serum and plasma contain a wide variety of substances that may interfere with ELISA outcomes, resulting in false positive data.

This application note from Bio-Techne features three examples of how the company’s proprietary blocking reagents help prevent false positive data in its Quantikine ELISAs as well as that of competitors.

Learn about the importance of optimization in assay development and how Bio-Techne’s in-house testing and careful selection of antibodies, proteins, and diluents help ensure your ELISAs will perform with optimum precision, specificity, accuracy, and sensitivity.

4 Fundamentals of Immunoassay Quality

White Paper

Immunoassay kits can be convenient time-saving tools, but not all assays are created equal. This white paper from Bio-Techne provides a detailed review of the core elements that form the foundation of immunoassay quality and their role in helping to deliver robust, reproducible, and reliable results.

The white paper examines each of these component – recovery, linearity, specificity, and consistency – in detail. It also outlines the strict procedures and protocols Bio-Techne applies to make sure each assay delivers superior performance and consistency as part of a stringent quality control program.

Luminex Assay Users Guide

White Paper

While measuring multiple analytes in a single sample offers numerous advantages, achieving optimum immunoassay outcomes requires careful consideration of multiple factors.

The Luminex Assay Users Guide provides a detailed overview of the performance capabilities and wide-ranging benefits of Luminex multiplex immunoassays. A detailed Q&A section provides answers to common questions related to Luminex performance, preparation protocols, and sample types.

ELISA Guide: A Clear and Easy Guide to ELISAs

White Paper

Your results matter. That is why choosing the right ELISA is critical for your research success.

The ELISA Guide from Bio-Techne provides a deep-dive look at the different types of ELISAs, advantages and disadvantages of each, and why you would want to use an ELISA over other techniques.

The guide also details the importance of ELISA controls and offers best practice recommendations for achieving optimum performance, precision, and reproducibility.

10 Strategic Considerations When Outsourcing Production of In Vitro Diagnostics

White Paper

This white paper highlights 10 important benefits of outsourcing and key considerations that should factor into the evaluation of an IVD  partner, from investment to development capabilities.

Life Sciences & Biotechnology Labeling Dilemmas

White Paper

Specifications for laboratory labels for biotech and diagnostics face unique demands, such as precise sizing, legibility, durability, and increasing information requirements, not to mention FDA compliance.

This white paper includes five case studies illustrating solutions for various labeling challenges for life science and biotech companies, including:

  • Chain of Custody Security & Traceability
  • Durability in Harsh Environments
  • Automatic Application Lines
  • Durability in Extreme Temperatures
  • Legibility & Sizing

LGC, Biosearch Technologies Accelerate Probe and Primer Production to Enable COVID-19 Testing with the SP Genevac HT Series 3i Evaporator

White Paper

During the COVID-19 pandemic, LGC, Biosearch Technologies, has been accelerating its production of oligonucleotides and other critical test kit components. However, there are problems that can slow production workflow. One particular bottleneck is in the drying process. The output from the synthesis labs and purification group can far outweigh the aliquoting, loading and drying workflow, which causes workflow to slow down upstream.

LGC, Biosearch Technologies, is using SP Genevac technology in order to facilitate the rapid production of probes and primers, and creating them to the high standards needed in this extremely challenging situation.

Download this white paper to learn how Biosearch Technologies used the SP Genevac technology to step up to the COVID-19 testing challenge.

Using Parallel Evaporators to Escalate COVID-19 Test Kit Preparation

White Paper

As labs globally have scaled up production of PCR test kits for COVID-19, workflow improvements continue to be important to increase the efficiency of test kit production.

SP Genevac's HT Series3i evaporator range offers a solution to improving the efficiency of test kit production, by preventing bottlenecks in high-throughput processing of oligonucleotides. It provides a fast and reliable method for drying primers and probes ready for use in PCR testing kits.

Download this white paper to learn how SP Genevac supported laboratories around the world to scale up their production of PCR test kits to aid the global fight against the COVID-19 virus.

Freeze-Drying COVID-19 Diagnostics: Formulation & Process Development and Lyophilizer Selection

White Paper

Lyophilization may be considered a relatively gentle drying process, but there are still risks and pitfalls when applying it to biomolecular reagents in diagnostic tests. Reagents typically used in ELISA- and PCR-based diagnostic kits for diseases such as COVID-19 tend to contain labile components, such as enzymes that need preserving for longevity in the supply chain or antibodies to test for a patients response to the virus, which can be challenging to stabilize for commercial use.

This technical note discusses various aspects of formulation and cycle development in the lyophilization of diagnostic reagents, including those required for COVID-19 testing.

PCR Diagnostic Kits & Freeze-Drying Considerations

White Paper

Polymerase chain reaction (PCR) is a method widely used in molecular biology to make many copies of a specific DNA segment. There are numerous processing challenges with PCR, however. The various component solutions need to be accurately combined in a plate well or tube "cocktail." The components require refrigeration and they have a relatively short shelf life and cross contamination is a concern. R&D lab bench pipetting errors can also be troublesome.

Freeze-drying has become an integral part of the PCR process and is used for both individual reagents as well as end-product test kit reagents.

This technical note discusses the considerations for freeze-dried PCR reagents and diagnostic kits.

Optimized Lateral Flow Assays Using Dyed and Fluorescent Estapor Microspheres

Video

In this technical presentation, we will discuss the use of polymeric microspheres in diagnostic assays. We will provide you with a survey of the materials used in this field, examples of readers available for this growing market, case studies, and a first preview of novel products to be released in 2020.  Material considerations to be discussed include key attributes needed to select the best spheres for qualitative and quantitative assays (such as size, functional group, and color).  We will also share best practices to troubleshoot protein binding in qualitative assay development using our raw materials. This information will be of interest to individuals involved in developing and validating qualitative and quantitative assays.

Applying AnteoBind Activated Estapor Europium Microspheres for Development of Fluorescent Lateral Flow Immunoassays

Video

In this technical presentation, we will provide you with an introduction to AnteoBind nanocoating technology and the use of AnteoBind activated Estapor europium microspheres in lateral flow immunoassays.

We will discuss the common issues encountered in antibody conjugation, the detail of the alternative protein conjugation procedure using AnteoBind activated Estapor europium microspheres, and the effects of physical characteristics (size, dispersity, surface charge) during the steps of conjugation and storage. We will also discuss the performance of conjugates in functional assay development for several popular biomarkers.

We will share best practices to troubleshoot antibody conjugation to AnteoBind activated Estapor europium microspheres in assay development and the initial scale-up procedure. This information will be of interest to individuals involved in developing and validating lateral flow immunoassays and manufacturing process development.