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November 19, 2019
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Stilla

Digital PCR for Tag Copy Number Assessment in CRISPR-Edited Cell Lines

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Fluorescent proteins or self-labeling tags are invaluable tools for studying protein dynamics in living cells using fluorescence microscopy. However, quantitative imaging requires physiological levels of expression of the target protein of interest (POI), especially when stoichiometric interactions of the POI need to be investigated.

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