This webinar will demonstrate how a research team at the Firestone Institute for Respiratory Health at McMaster University developed a cellular and molecular phenotyping pipeline using archived samples of lung tissue derived from patients diagnosed with fibrotic interstitial lung disease.
Dr. Kjetil Ask will discuss how the clinically relevant tissues were obtained, clinically categorized and deidentified. He will provide his perspective on the requirement of cross-disciplinary expertise and the associated technological platform required for the identification and quantification of cellular and molecular targets in fibrotic lung disease. Dr. Ask will also discuss the potential application of this platform to pre-clinical models of lung disease.
Megan Vierhout will present her research on the role of alternatively activated macrophages and endoplasmic reticulum stress in fibrotic lung disease. She will show specific examples of automated immunohistochemistry and associated in situ hybridization technology using ACD RNAScope and BaseScope assays to demonstrate molecular phenotyping and target identification.